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KMID : 0861020200350040045
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Korea Journal of Herbology
2020 Volume.35 No. 4 p.45 ~ p.50
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Single Oral Dose Toxicity Study of Black Raspberry Extractin Sprague-Dawley Rats
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Lee Joo-Young
Ji Kon-Young
Song Kwang-Hoon
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Abstract
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Objective : This study was performed to evaluate the toxicity after a single oral administration of black raspberry extract to male and female Sprague-Dawley (SD) rats and to determine the approximate lethal dose (ALD).
Methods : We previously showed that the black raspberry extract repressed the simvastatin-mediated expression of Proprotein convertase subtilisin/kexin type 9 (PCSK9) and improved Low-Density Lipoprotein cholesterol (LDL-C) uptake by hepatocytes through the induction of the Low-Density Lipoprotein Receptor expression in hepatocytes. The groups consisted of black raspberry extract groups, as an oral dose of 2,000 ㎎/㎏ and a control group. 5 weeks SD rats were randomly assigned to 4 groups of 5 rats. Each male and female SD rats were administered orally once. For 14 days after the administration, mortality, clinical signs, changes in body weight, and necropsy findings were observed according to the “Standard for Toxicity Study of Pharmaceuticals” of Korea Food and Drug Administration (KFDA) guideline and “Acute Oral Toxicity? Fixed Dose Procedure” of OECD Test Guideline.
Results : There were no cases of mortality in the group administered with 2,000 ㎎/㎏ of male and female, and no abnormalities in body weight change and clinical signs. Also, no gross abnormalities were observed at the autopsy.
Conclusions : As a result of a single oral administration of the black raspberry extract to SD rats, the ALD was determined to exceed 2,000 ㎎/㎏ for both male and female SD rats.
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KEYWORD
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Black raspberry, Rubus coreanus Miquel, Single oral dose toxicity
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